Building on last week's 2-1 comeback win in Chile, Brazil looked livelier from the start and dominated possession, with late goals from Andreas Pereira and Luiz Henrique putting the gloss on an impressive performance.
Making only his second international appearance since recovering from an injury sustained at the Copa America in July, Messi capitalised on a mistake by defender Marcelo Suarez to open the scoring in the 19th minute.
Reigning world champions Argentina remain top with 19 points, holding a three-point lead over Colombia, despite being held to a 1-1 draw by Venezuela in their previous match, where Lionel Messi returned from injury.
Real's Militao, who also missed last month's international fixtures with a leg injury, had travelled to Sao Paulo after featuring as a late substitute in Real's 2-0 win over Villarreal on Saturday.
Vinicius suffered a neck injury during Real Madrid's 2-0 home win against Villarreal on Saturday, the club said.
Messi, who plays for Major League Soccer club Inter Miami, was left out of Argentina's squad for their World Cup Qualifiers against Chile and Colombia in August due to the injury which he sustained during the Copa America final.
The reigning world champions will also be without Angel Di Maria after the veteran winger's international retirement.
The defeat saw Bangladesh finish at the bottom of four-team group with a single point from six matches.
The Bangladesh football team are trying to acclimatise to the scorching heat in Qatar, where they are set to take on Lebanon in their final match in the FIFA World Cup qualifiers at the Khalifa International Stadium in Al Rayyan on Tuesday.
Building on last week's 2-1 comeback win in Chile, Brazil looked livelier from the start and dominated possession, with late goals from Andreas Pereira and Luiz Henrique putting the gloss on an impressive performance.
Making only his second international appearance since recovering from an injury sustained at the Copa America in July, Messi capitalised on a mistake by defender Marcelo Suarez to open the scoring in the 19th minute.
Reigning world champions Argentina remain top with 19 points, holding a three-point lead over Colombia, despite being held to a 1-1 draw by Venezuela in their previous match, where Lionel Messi returned from injury.
Real's Militao, who also missed last month's international fixtures with a leg injury, had travelled to Sao Paulo after featuring as a late substitute in Real's 2-0 win over Villarreal on Saturday.
Vinicius suffered a neck injury during Real Madrid's 2-0 home win against Villarreal on Saturday, the club said.
Messi, who plays for Major League Soccer club Inter Miami, was left out of Argentina's squad for their World Cup Qualifiers against Chile and Colombia in August due to the injury which he sustained during the Copa America final.
The reigning world champions will also be without Angel Di Maria after the veteran winger's international retirement.
The defeat saw Bangladesh finish at the bottom of four-team group with a single point from six matches.
The Bangladesh football team are trying to acclimatise to the scorching heat in Qatar, where they are set to take on Lebanon in their final match in the FIFA World Cup qualifiers at the Khalifa International Stadium in Al Rayyan on Tuesday.
“I always said the result was not that important to me. The objective was to improve the performance, which we did not do in Melbourne and I think we achieved that objective regardless of the result,” said Bangladesh coach Javier Cabrera after the match.